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These elevations may also contribute to enhanced downstream signalling of Gq through activation of phospholipase C (PLC), as has been reported in INS-1 cells [22]

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After US treatment for 2 min (1.5 W cm 2 , 1 MHz, duty ratio 50%), the original culture medium was replaced with a free-medium-included DCFH-DA fluorescent probe (1:1000) and incubated at 37 C for 30 min

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